Answer

Which types of assays might make high resolution necessary?

With spots in all size categories, high resolution images provide more information on the spot morphology, thereby increasing the accuracy of the advanced spot morphology recognition features offered by ImmunoSpot® software (SmartSpot™). The spot size reflects the per cell productivity and secretion kinetics. Also, the strength of T cell stimulation defines the spot size; low avidity T cell recognition results in low productivity and small, faint spots. Granzyme B, TNF and IL-10 assays frequently produce small spots. In all these cases, using high-resolution image analysis can provide a definite advantage.

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