Manage Your Data with SpotMap™
An Indispensible Data Management Tool for High-Volume ELISPOT Data Processing
SpotMap™ was especially developed to increase productivity in routine laboratory settings by minimizing the workload and reducing the potential for human error in assay management.
Information on patients, antigens, reagents and different test conditions create a flood of interrelated data.
Link them all to the assay results with SpotMap™
SpotMap™: Software for ELISPOT Data Management
Because Elispot assays require few cells and relatively little labor, they readily lend themselves to high-throughput work. A single scientist may conduct a 20-plate experiment while a well-trained team can run as many as 200 plates per day. Even a small two-plate experiment would encompass 192 wells. In keeping with the principles of good laboratory practice, the raw images need to be saved, as do the results of the automated counting. In addition, some wells may need to be recounted if they are damaged or contain artifacts, and these changes must likewise be documented. Thus, with over 400 image files and much more information involved, even a small two-plate experiment would benefit from streamlined data management. Born in the laboratory and matured to meet the day to day requirements of ELISPOT work, the ImmunoSpot® portfolio meets this need.
Our SpotMap™ software permits researchers to structure their experiments from the early planning phase to the final statistical evaluation of the results. Plan your experiments in SpotMap™ and see the difference!
The first step in SpotMap™ is to create a database with the test sample and reagent information. Then, one defines the layout for all plates, specifying which cells are to be tested and in what numbers, along with the antigens/peptides to be plated and their concentrations.
The software even helps in planning experiments by calculating the quantities of antigen, coating, capture antibodies and cells required.
Update Your Protocols in SpotMap™
By the very nature of science, experimental reality might not follow the plan. For example, you might intend to do a 36-well experiment with each sample, but after thawing the cryopreserve PBMC, find that some of the samples lack enough viable cells. You therefore decide to test these samples in duplicates, not the initially planned triplicates, or to skip antigens. Just mark in SpotMap™ which wells on what plates did not contain cells and when it comes to the final evaluation, SpotMap™ will automatically take these changes into account.
Finalize Your Data in SpotMap™
After the ELISPOT plates have been developed and scanned, they are counted and quality controlled using the ImmunoSpot® software. SpotMap™ permits you to import the spot counts and to overlay them with all the information contained in the plate design. The counts for each well will be assigned to the respective test subject, antigen and cytokine (see figure). For replicate wells, it calculates means, standard deviations, or medians among multiple statistical options. It also permits the subtraction of background counts. Without SpotMap™, these final steps alone could require a full day in a 20-plate experiment. With SpotMap™, it takes mere seconds.