BioSpot™ Software Suite

Neutralization assays and colonies counting

Immune monitoring efforts frequently involve measuring serum antibodies' efficacy in neutralizing viruses, bacteria or toxins. ImmunoSpot® readers fully support such high throughput measurements, including colony counting in any well format (including Petri dish).

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Here an overview of viral assays counted by ImmunoSpot® Readers:

Viral titer
Plaque Reduction Neutralization Assay (PRNT)
Virus/serum neutralization
Viral immune-colorimetric assay (ICA)
Focus forming assay
Intracellular inclusion assay
Plaque Forming Unit / Focus-Forming Unit (PFU/FFU) assay
Cytopathic Effect (CPE)

For examples of ImmunoSpot® Readers' use in SARS-CoV-2 assays follow this link.

For a key multi-color reference utilizing an ImmunoSpot® Reader for viral applications also see J. Immunol., 2015, 195: 3490.

ImmunoSpot® Readers excel in high throughput bacterial colony counting, in any well format, from 384 to Petri dish and square agar slabs.

Serum bactericidal assays (SBA) and
Opsono-Phagocytotic assays (OPK) are commonly counted by ImmunoSpot® Readers

For a key reference utilizing an ImmunoSpot® Reader for bacterial colony counting see J. Immunol. Methods. 2004, 292: 187.

ImmunoSpot® Readers are also uniquely suited for high throughput yeast colony counting, in any well format, from 384 to Petri dish, as applies to:

Humanized yeast models
Proteomics (Y2H)
Antibiotic testing
Toxicology

For a key reference utilizing an ImmunoSpot® Reader for such applications see Cell Death & Disease, 2011, 2:188

High throughput is of essence for colony counting. In an optimized configuration, an ImmunoSpot® Reader can count a 384 well plate in less than one minute, with 400 plates in a single run.

Learn More about our plaque colony counting.