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The latest news from CTL

Confirm instrument performance with a reference plate and dedicated ImmunoQualify software – generate Pass/Fail reports with a click of a button.

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Our IntelliCount software uses spot recognition criteria that are independent of some of the variables commonly affecting classical counting results such as differences in light sources, optics and other image capture components . This software is a major step towards elevating ELISpot to an exact science by coming up with consistent/standardized counts.

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Live/dead cell counting is important for the assessment of PBMC samples’ fitness following cryopreservation or prolonged storage/shipment [ref]. The accurate counting of live cells in PBMC is essential for adjusting the cell numbers to be plated into ELISPOT/FluoroSpot assays as divergent cell counts contribute the largest variability to inter-assay reproducibility. Unless using a dedicated instrument for live/dead cell counting, this process is time-consuming and rate limiting for testing of multiple PBMC samples in an experiment. No longer!

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Measuring cell-mediated cytotoxicity is part of the assay repertoire of most laboratories involved in immune monitoring efforts. CTL’s philosophy is to offer instruments that support several functions commonly performed in immune monitoring laboratories. Enabled by our TVA Software suite, CTL’s fluorescence capable ImmunoSpot® instruments can measure cell-mediated cytotoxicity.

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The primary function of SpotStat® is to provide statistically-validated discrimination between antigen-induced responses and negative controls. SpotStat® was specifically designed to perform multiple comparison tests using a number of family-wise error correction methods, when multiple positive responses (e.g. peptide screening in T cell assays) are compared to a single negative control for the same subject (donor).

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No instruments needed, no PBMC or antigens needed, we provide it all! You can get started any time.

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Membranes of PVDF plates suited for ELISPOT/FluoroSpot analysis can show regional defects that can cause individual wells to fail. No longer! At CTL, for our ImmunoSpot® kits we now quality control the membrane of each well, and we devised ways to quality control the even pre-coating of each well, in addition.

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The number of pipetting steps and incubation times were reduced for fast results.

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You may not want to miss this opportunity if you are studying in humans the effect of any type of genetic variation on any type of cellular function.

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Looking for positive/negative control PBMC reference samples? In addition to offering PBMC that have defined numbers of antigen-specific T cells, we now also offer PBMC with defined numbers of antigen-specific B cells.

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Conventional FluoroSpot relies on the analysis of a single image of a well, captured using a single exposure level. This approach may miss faint spots, while overly bright spots can be over-exposed, leading to inaccurate quantitation of the per cell secretory activity. No longer!

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Our recent publication details how comprehensive ImmunoSpot® testing against all potential determinants of an antigen is technically feasible, and calls into question the usage of single or few previously established or predicted peptides for reliable immune monitoring.

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