Human IFN-γ/Granzyme B/IL-17 Three-Color FluoroSpot
This optimized FluoroSpot assay reliably detects and measures human IFN-γ, GzB and IL-17 secretions by stimulated effector cells in frequencies as low as one in a million.
Scanning and analysis requires an analyzer with fluorescent capabilities. CTL recommends any of the following Series 5 or Series 6 readers running ImmunoSpot® Software version 5.3 or higher: S5UV, FluoroCore, Ultra, Universal, Ultimate. Please contact us before placing your order if you are uncertain about your setup.
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Protocol Request
Protocol Request
Pricing is for US and Canada only and does not include shipping.
All others, contact the distributor in your region.
A flat rate for shipping will be applied to all orders. To use your company's FedEx account number, please contact us before placing your order.
Details
ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!
Capture Kit
Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.
Detection
Kit Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.
Volume discounts available, please inquire.
Components | Catalog # |
---|---|
IFN-γ/GzB/IL-17 Capture Kit | hT3017F |
IFN-γ Detection Kit | hT02 |
GzB Detection Kit | hT08 |
IL-17 Detection Kit | hT32 |
Related Products & Services
Documents
- USPA (3.52 MB)
- Four-color T and B cell ELISPOT for simultaneous detection of analytes (266.46 KB)
- ImmunoSpot T Cell Kits Brochure (359.99 KB)
- ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring — even in hands of ELISPOT-inexperienced investigators (312.75 KB)
- High reproducibility of ELISPOT counts from nine different laboratories (534.74 KB)
- ELISPOT assays in 384-well format: Up to 30 data points with one million cells (374.30 KB)
- Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT assays (701.83 KB)
- Optimal thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies (237.77 KB)
- Neuroantigen-specific CD4 cells expressing IFN-γ, IL-2 and IL-3 in a mutually exclusive manner prevail in EAE (572.39 KB)
- Terms and Conditions (103.69 KB)
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