Human IgA/IgE Double-Color FluoroSpot

This optimized Fluorospot assay reliably detects human IgA and IgE secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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Details

This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture/detections antibodies and colorimetric substrates.

Capture Kit

Includes low autofluorescence PVDF plates and all diluents.

Detection Kits

Kits contain unlabeled and/or fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.

Components Catalog #
Ig Capture Kit hB2F
IgA Detection Kit hB18
IgE Detection Kit hB19

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