This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.

Mouse IFN-γ/IL-4/IL-5 Three-Color FluoroSpot

The ImmunoSpot IFN-γ/IL-4/IL-5 assay is the first ever triple color mouse FluoroSpot.

Detect cells secreting each cytokine individually or in combination(s) with accuracy comparable to single-color (single cytokine) analysis.

Stimulated effector cells can be detected in frequencies as low as one in a million, allowing the enumeration of rare polyfunctional cells.

Analysis of the ImmunoSpot triple color FluoroSpot requires a fluorescence capable analyzer with the appropriate filters and light sources. CTL recommends any of the following Series 6 analyzers: FluoroCore, Ultra, Universal, Ultimate. (ImmunoSpot v7.0 or higher software is recommended for accurate analysis). Contact us now to ensure your analyzer has the proper configuration, or inquire about our Plate Scanning Services.

More Information
Components Catalog # Per Plate $
Capture Kit mT3005F $285.00
IFN-γ Detection Kit mT02 $40.00
IL-4 Detection Kit mT38 $40.00
IL-5 Detection Kit mT41 $40.00


ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit

Includes Capture Antibody for each cytokine plus serum-free medium, low autofluorescence PVDF plates, and all diluents.


Kit Contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted cytokine of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific cytokine by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.