This optimized FluoroSpot assay reliably detects mouse IgG1, IgG2a, IgG3 and IgM produced by antibody-secreting cells (ASC) that have either differentiated in vivo or were induced to differentiate into ASC following polyclonal stimulation in vitro.
ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!
Includes Capture Antibodies, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.
Kit contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.