Mouse IgG1/IgG2c Double-Color ELISPOT

This optimized ELISPOT assay reliably detects mouse IgG1 and IgG2c secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-S™) capable of eliciting IgG1/IgG2c antibody-secreting cells (ASC).