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Mouse IgG2b/IgG3 Double-Color FluoroSpot

This optimized Fluorospot assay reliably detects mouse IgG2b and IgG3 secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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Details

ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!

Capture Kit

Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.

Detection

Kit contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.

Volume discounts available, please inquire.

Components Catalog #
Ig Capture Kit mB2F
IgG2b Detection Kit mB37
IgG3 Detection Kit mB50

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