Mouse IgG2b/IgM Double-Color ELISPOT

This optimized ELISPOT assay reliably detects mouse IgG2b and IgM secretions from either Antibody Secreting Cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.

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This ImmunoSpot® kits contains PVDF-membrane plates, diluents, capture antibody, detection reagents, colorimetric substrates and polyclonal B cell stimulator (B-Poly-S™) capable of eliciting IgG2b/IgM antibody-secreting cells (ASC).