PBMC Sampling Sets
Details
Our Cryopreserved PBMC are ideal for the following applications:
- ELISPOT/FluoroSpot Assays
- Tetramers/Pentamers/Dextramers
- Intracytoplasmic Cytokine Staining (ICS)
- Cytokine ELISA Assays
- Cytokine Bead Arrays (CBA)
- Cytokine Protein Arrays (CPA)
- Cytokine mRNA Determinations (RT-PCR)
- ADCC
- Transmigration Assays
- Any applications that require live, functional PBMC
Documents
- Characterized ePBMC® Product Data Sheet (424.93 KB)
- USPA (3.52 MB)
- Protocols-Guidelines for PBMC (254.16 KB)
- Protocol (114.68 KB)
- CTL ePBMC Brochure (275.24 KB)
- Serum Free Media Brochure (209.90 KB)
- ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring — even in hands of ELISPOT-inexperienced investigators (312.75 KB)
- ELISPOT assays in 384-well format: Up to 30 data points with one million cells (374.30 KB)
- Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT assays (701.83 KB)
- Optimal thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies (237.77 KB)
- Terms and Conditions (103.69 KB)
Pooled PBMC from an even number of healthy male and female donors. All donors are prescreened and selected per the European Pharmacopoeia guidelines: no NSAIDs administered for one week and no steroids for one month prior to aphaeresis. Independent functionality tests were performed to measure responses to endotoxin, lipoteichoic acid and peptidoglycan by ELISA to ensure a high background to response ratio. Independent functionality tests have been performed to measure IL-6 responses to endotoxin, lipoteichoic acid and peptidoglycan by ELISA to ensure a high signal to background ratio. Independent functionality tests have been performed to measure IL-6 responses to endotoxin, lipoteichoic acid and peptidoglycan by ELISA to ensure a high signal to background ratio. Certificate of AnalysisAll processing media (CTL serum-free media) used during PBMC isolation and cryopreservation are tested for pyrogens. |
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