This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.

FCS output

Figure Legend:
Clonal B cell hybridoma lines secreting monoclonal antibody (mAb) with low- (P65C6-2.l, black dots), intermediate- (35-65.1, red dots) or high-affinity (36-71.3, blue dots) for a model antigen (p- azophenylarsonate = Ars) were assessed for FluoroSpot formation. FluoroSpots generated in wells coated with A) Ars-BSAint or B) Ars-BSAhigh were revealed using the Murine IgGl Single-Color FluoroSpot detection system and counted using ImmunoSpot 7 software. Counted FluoroSpots from replicate wells were then merged into a flow cytometry standard (FCS) output. Differential affinity of the anti-Ars mAb was confirmed through plotting the mean intensity and size of individual Fluorospots generated from the respective B cell hybridomas.