T Cell ELISPOT Testing
The ability to measure the response of T cells to antigens is vital for basic research, drug discovery, and management of a range of important diseases. ELISPOT testing is particularly useful in areas when measurement of low-frequency, T cell responses is required for the ex vivo readout of immune responses. The T cell readout at single-cell resolution is required to develop better understanding and treatment of allergies and autoimmunity, to predict rejection/acceptance responses in organ transplantation, for improvements in drug and vaccine development, for monitoring various cancer treatments and more.
The ELISPOT technique allows researchers to generate secretory footprints in the form of colored spots within a 96-well plate. These images can then be scanned and counted, and readily evaluated for statistical significance. The ELISPOT method can therefore be used to determine whether a patient/sample population responds positively or negatively to a vaccine, drug or biological product, and in addition, measure the extent of each person’s immune response. This test method opens the door for other means of data evaluation, such as:
- Testing T cell-mediated immunity at a single-cell level for pre-clinical and clinical specimens
- Determinant mapping with overlapping peptides
- Measurement of cytotoxic T cell responses
- The ability to run multiple assays using a limited number of cells/specimens
- High-throughput testing capacity (~150 specimens per day, ~450 specimens per week)
CTL’s invaluable expertise (see publications) encompasses a wide range of assay types, thanks to its experience in working with clients to optimize, qualify, and validate test methods for various test systems e.g. human, NHP, monkey, mouse, rat, pig, and more. Please see list of CTL's T cell ELISPOT Kits available as these possible T cell readouts are available to be used by our contract laboratory.