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This optimized Fluorospot assay reliably detects human TNF-α, IgG, and IgM secretions from total PBMC, isolated antibody-secreting cells that have been immunized/activated in vivo, or memory B cells that have been polyclonally stimulated in vitro to secrete antibody.
ImmunoSpot® offers the ONLY COMPLETE set of Kits which contain everything you need — ALL FROM ONE SOURCE!
Capture Kit
Includes Capture Antibodies, serum- free medium, low autofluorescence PVDF plates, polyclonal B cell activator (B-Poly-S™), and all diluents.
Detection
Kit contains fluorescently-labeled Detection Antibodies and fluorochrome conjugates that bind to the secreted antibodies of interest. The fluorochrome conjugates contain proprietary dye systems for fluorescence detection of the specific Ig class/IgG subclass by scanning and analyzing the emitted wavelength of the fluorochrome(s) using an ImmunoSpot® fluorescent Analyzer.
